Solution NMR chemical shift assignment of apo and molybdate-bound ModA at two pHs.

ModA is a soluble periplasmic molybdate-binding protein found in most gram-negative bacteria. It is part of the ABC transporter complex ModABC that moves molybdenum into the cytoplasm, to be used by enzymes that carry out various redox reactions. Since there is no clear analog for ModA in humans, this protein could be a good target for antibacterial drug design. Backbone 1H, 13C and 15N chemical shifts of apo and molybdate-bound ModA from E. coli were assigned at pHs 6.0 and 4.5. In addition, side chain atoms were assigned for apo ModA at pH 6.0. When comparing apo and molybdate-bound ModA at pH 6.0, large chemical shift perturbations are observed, not only in areas near the bound metal, but also in regions that are distant from the metal-binding site. Given the significant conformational change between apo and holo ModA, we might expect the large chemical shift changes to be more widespread; however, since they are limited to specific regions, the residues with large perturbations may reveal allosteric sites that could ultimately be important for the design of antibiotics that target ModA.

Safety assessment of mixtures of 1,9-nonanediamine (NMDA) and 2-methyl-1,8-octanediamine (MODA), for use in food contact materials.

The EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) assessed the safety of mixtures of 1,9-nonanediamine (NMDA) and 2-methyl-1,8-octanediamine (MODA) when used to produce polyamide food contact materials for contact with all food types for long-term storage at room temperature and below, including heating up to 121°C for up to 2 h. The polyamide material is also intended to be used for repeated use up to 121°C with short contact (up to 30 min). The polymer typically contains ■■■■■ of a low molecular weight fraction (LMWF, < 1000 Da). The specific migration was measured with polyamide samples in a set of migration tests with 3% acetic acid and 10% ethanol. NMDA and MODA were not detected at ■■■■■, respectively. The specific migration of the LMWF consisting of NMDA/MODA-related species was up to ■■■■■. The overall migration in olive oil was below the detection limit (3 mg/dm2). The most abundant migrating LMWF oligomers were identified. Toxicological studies were performed with NMDA, MODA and with polyamide formulations enriched in the LMWF. The results of genotoxicity assays did not raise a concern. From a repeated-dose oral 90-day toxicity study in rats, the Panel identified a no observed adverse effect level (NOAEL) of 1000 mg/kg body weight per day for the migrating LMWF. The CEP Panel concluded that NMDA/MODA mixtures do not raise a safety concern for the consumer when used as comonomer with terephthalic acid to manufacture polyamide articles intended for contact with all food types, except for infant formula and human milk, if the migration of NMDA and MODA does not exceed 0.05 mg/kg food (as a sum of the two substances) and if the migration of the LMWF consisting of NMDA/MODA-related species does not exceed 5 mg/kg food.

Prevalence and factors associated with multidimensional child deprivation: Findings from the Future of Families and Child Well-Being Study.

Despite the growing importance of the multidimensional methods of assessing child poverty, few studies in the U.S. have applied a rights-based approach to examining child deprivation. This study examines multidimensional child deprivation using eight dimensions and twelve indicators based on the Convention on the Rights of the Child (CRC). Using a sample of children at age nine from the fifth wave of the Future of Families and Child Well-being Study, this study applied the multiple overlapping deprivation analysis (MODA), a comprehensive analytic method to assess the multidimensionality of child deprivation and to provide a detailed picture of material and social forms of deprivation among the U.S. children. This study found that the overall child deprivation rate was 8.89%; environmental safety (20.36%), information (15.94%), and housing security (14.23%) dimensions contributed the highest to the overall child deprivation; the overlap between deprivation and income poverty was 12.83%. Results suggest that understanding multifaceted and interrelated contexts of child deprivation is crucial to promote child rights.

Structural analysis of molybdate binding protein ModA from Klebsiella pneumoniae.

Klebsiella pneumoniae, a facultative anaerobe, relies on acquiring molybdenum to sustain growth in anaerobic conditions, a crucial factor for the pathogen to establish infections within host environments. Molybdenum plays a critical role in pathogenesis as it forms an essential component of cofactors for molybdoenzymes. K. pneumoniae utilizes the ABC (ATP-Binding-Cassette) transporter encoded by the modABC operon for uptake of the group VI elements molybdenum and tungsten. In this study, we determined the X-ray crystal structures of both the molybdenum-free and molybdenum-bound substrate-binding protein (SBP) ModA from Klebsiella pneumoniae to 2.00 Å and 1.77 Å resolution respectively. ModA crystallizes in the space group P222 with a single monomer in one asymmetric unit. The purified protein remained soluble and specifically bound molybdate and tungstate with Kd values of 6.3 nM and 5.2 nM, respectively. Tungstate competes with molybdate by binding to ModA, resulting in enhanced antimicrobial activity. These data provide a starting point for structural and functional analyses of molybdate transport in K. pneumoniae.

SARS-CoV-2 Omicron Subvariants Balance Host Cell Membrane, Receptor, and Antibody Docking via an Overlapping Target Site.

Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are emerging rapidly and offer surfaces that are optimized for recognition of host cell membranes while also evading antibodies arising from vaccinations and previous infections. Host cell infection is a multi-step process in which spike heads engage lipid bilayers and one or more angiotensin-converting enzyme 2 (ACE-2) receptors. Here, the membrane binding surfaces of Omicron subvariants are compared using cryo-electron microscopy (cEM) structures of spike trimers from BA.2, BA.2.12.1, BA.2.13, BA.2.75, BA.3, BA.4, and BA.5 viruses. Despite significant differences around mutated sites, they all maintain strong membrane binding propensities that first appeared in BA.1. Both their closed and open states retain elevated membrane docking capacities, although the presence of more closed than open states diminishes opportunities to bind receptors while enhancing membrane engagement. The electrostatic dipoles are generally conserved. However, the BA.2.75 spike dipole is compromised, and its ACE-2 affinity is increased, and BA.3 exhibits the opposite pattern. We propose that balancing the functional imperatives of a stable, readily cleavable spike that engages both lipid bilayers and receptors while avoiding host defenses underlies betacoronavirus evolution. This provides predictive criteria for rationalizing future pandemic waves and COVID-19 transmissibility while illuminating critical sites and strategies for simultaneously combating multiple variants.

Adherence of Nontypeable Haemophilus influenzae to Cells and Substrates of the Airway Is Differentially Regulated by Individual ModA Phasevarions.

Adherence of nontypeable Haemophilus influenzae (NTHi) to the host airway is an essential initial step for asymptomatic colonization of the nasopharynx, as well as development of disease. NTHi relies on strict regulation of multiple adhesins for adherence to host substrates encountered in the airway. NTHi encode a phase-variable cytoplasmic DNA methyltransferase, ModA, that regulates expression of multiple genes; a phasevarion (phase-variable regulon). Multiple modA alleles are present in NTHi, in which different alleles methylate a different DNA target, and each controls a different set of genes. However, the role of ModA phasevarions in regulating adherence of NTHi to the host airway is not well understood. This study therefore sought to investigate the role of four of the most prevalent ModA phasevarions in the regulation of adherence of NTHi to multiple substrates of the airway. Four clinical isolates of NTHi with unique modA alleles were tested in this study. The adherence of NTHi to mucus, middle ear epithelial cells, and vitronectin was regulated in a substrate-specific manner that was dependent on the ModA allele encoded. The adhesins Protein E and P4 were found to contribute to the ModA-regulated adherence of NTHi to distinct substrates. A better understanding of substrate-specific regulation of NTHi adherence by ModA phasevarions will allow identification of NTHi populations present at the site of disease within the airway and facilitate more directed development of vaccines and therapeutics. IMPORTANCE Nontypeable Haemophilus influenzae (NTHi) is a predominant pathogen of the human airway that causes respiratory infections such as otitis media (OM) and exacerbations in the lungs of patients suffering from chronic obstructive pulmonary disease (COPD). Due to the lack of a licensed vaccine against NTHi and the emergence of antibiotic-resistant strains, it is extremely challenging to target NTHi for treatment. NTHi adhesins are considered potential candidates for vaccines or other therapeutic approaches. The ModA phasevarions of NTHi play a role in the rapid adaptation of the pathogen to different environmental stress conditions. This study addressed the role of ModA phasevarions in the regulation of adherence of NTHi to specific host substrates found within the respiratory tract. The findings of this study improve our understanding of regulation of adherence of NTHi to the airway, which may further be used to enhance the potential of adhesins as vaccine antigens and therapeutic targets against NTHi.

Linhas, alinhavos e descosturas no processo de criação de uma performance/exposição / Líneas, hilvanados y costuras en el proceso de creación de una actuación/exposición / Lines, bastings and seams in the process of creating a performance/exhibition

Resumo É objetivo deste artigo analisar o processo de criação de Fernanda Yamamoto e sua equipe, tendo como foco a criação da performance/exposição para a primeira edição presencial pós-pandemia do evento de moda São Paulo Fashion Week, ocorrida em novembro de 2021. Apoiadas nas contribuições de Lev Vigotski, Mikhail Bakhtin, Cecília Salles e Suely Rolnik, entre outros/as, as discussões propõem compreender a prática artística e as manualidades como possibilidade de instaurar caminhos de reinvenção dos elementos da vida, de si mesmo e do mundo. Conclui-se que os modos de criar instituídos pela artista e sua equipe constituem o ateliê como espaço em que é possível visibilizar as tensões próprias do sistema capitalista ao mesmo tempo em que forjam desvios potencializadores, expressando modos outros de produção da realidade e da subjetividade na contemporaneidade.

Resumen El objetivo de este artículo es analizar el proceso de creación de Fernanda Yamamoto y su equipo, centrándose en la creación de la actuación/exposición para la primera edición presencial pospandemia del evento de moda São Paulo Fashion Week, que tuvo lugar en noviembre de 2021. Con aportes de Lev Vigotski, Mikhail Bakhtin, Cecília Salles y Suely Rolnik, entre otros, los debates proponen entender la práctica artística y la artesanía como una posibilidad para establecer formas de reinventar los elementos de la vida, de uno mismo y del mundo. Se concluye que las formas de creación establecidas por la artista y su equipo constituyen el estudio como un espacio en el que es posible visibilizar las tensiones del sistema capitalista al mismo tiempo que forjan desviaciones potenciadoras, expresando otros modos de producción de realidad y subjetividad en la contemporaneidad.

Abstract The aim of this article is to analyze the creation process of Fernanda Yamamoto and her team, focusing on the creation of the performance/exhibition for the first post-pandemic in-person edition of the fashion event São Paulo Fashion Week, which took place in November 2021. Supported by the contributions from Lev Vigotski, Mikhail Bakhtin, Cecília Salles and Suely Rolnik, among others, the discussions propose to understand the artistic practice and handicrafts as a possibility to establish ways of reinventing the elements of life, the self and the world. It is concluded that the ways of creating established by the artist and her team constitute the studio as a space in which it is possible to make visible the tensions of the capitalist system at the same time that they forge potentiating deviations, expressing other modes of production of reality and subjectivity in contemporaneity.

An Important Role of the Type VI Secretion System of Pseudomonas aeruginosa Regulated by Dnr in Response to Anaerobic Environments.

The type VI secretion system (T6SS) is capable of secreting a variety of metal-binding proteins involved in metal ion uptake, and it mediates an active metal ion transport system that contributes to competition between bacteria. Pseudomonas aeruginosa H2-T6SS can increase molybdenum ion acquisition and enhance bacterial survival advantage by promoting the secretion of the molybdate-binding protein ModA, in which the expression of H2-T6SS core genes hcp2, hsiA2, and clpV2 is activated by anaerobic conditions and are all regulated by the global regulator Anr. Here, we report the regulation of T6SS by Dnr, a dedicated dissimilatory nitrate respiration regulator in P. aeruginosa. Of the three distinct T6SS loci carried by P. aeruginosa, only the anaerobic expression of H2-T6SS was activated by Dnr; H1-T6SS or H3-T6SS did not respond to anaerobically induced activation. We also demonstrated that Dnr promotes the anaerobic secretion of ModA, which acts as a potential substrate for H2-T6SS, providing an advantage not only for the anaerobic growth of bacteria but also for functional competition. Overall, this study elucidates the important role played by Dnr in mediating the anaerobic expression of T6SS in P. aeruginosa, indicating that the functional advantage of H2-T6SS in response to anaerobic induction may be a conditional environmental adaptation. It also extends our understanding of the function of Dnr as a specific regulator of dissimilatory nitrate respiration. IMPORTANCE The type VI secretion system (T6SS) plays an important role in bacterial competition by mediating the transport of active metal ions. Pseudomonas aeruginosa carries three distinct T6SS loci (H1-, H2-, and H3-T6SS). The H2-T6SS promotes the secretion of the molybdate-binding protein ModA for the acquisition of molybdenum ions to adapt to anaerobic survival. Here, we report that the specialized dissimilatory nitrate respiration regulator Dnr in P. aeruginosa controls the anaerobic expression of H2-T6SS and that this regulation is essential for ModA protein secretion, anaerobic growth, and bacterial competition. This study elucidates the regulatory mechanism of Dnr on H2-T6SS in P. aeruginosa, revealing an important role played by H2-T6SS in adapting to an anaerobic environment.

The Impact of Chromate on Pseudomonas aeruginosa Molybdenum Homeostasis.

Acquisition of the trace-element molybdenum via the high-affinity ATP-binding cassette permease ModABC is essential for Pseudomonas aeruginosa respiration in anaerobic and microaerophilic environments. This study determined the X-ray crystal structures of the molybdenum-recruiting solute-binding protein ModA from P. aeruginosa PAO1 in the metal-free state and bound to the group 6 metal oxyanions molybdate, tungstate, and chromate. Pseudomonas aeruginosa PAO1 ModA has a non-contiguous dual-hinged bilobal structure with a single metal-binding site positioned between the two domains. Metal binding results in a 22° relative rotation of the two lobes with the oxyanions coordinated by four residues, that contribute six hydrogen bonds, distinct from ModA orthologues that feature an additional oxyanion-binding residue. Analysis of 485 Pseudomonas ModA sequences revealed conservation of the metal-binding residues and ß-sheet structural elements, highlighting their contribution to protein structure and function. Despite the capacity of ModA to bind chromate, deletion of modA did not affect P. aeruginosa PAO1 sensitivity to chromate toxicity nor impact cellular accumulation of chromate. Exposure to sub-inhibitory concentrations of chromate broadly perturbed P. aeruginosa metal homeostasis and, unexpectedly, was associated with an increase in ModA-mediated molybdenum uptake. Elemental analyses of the proteome from anaerobically grown P. aeruginosa revealed that, despite the increase in cellular molybdenum upon chromate exposure, distribution of the metal within the proteome was substantially perturbed. This suggested that molybdoprotein cofactor acquisition may be disrupted, consistent with the potent toxicity of chromate under anaerobic conditions. Collectively, these data reveal a complex relationship between chromate toxicity, molybdenum homeostasis and anaerobic respiration.

Moda e trabalho como prática de exclusão social: uma revisão das produções acadêmicas brasileiras / Fashion and Work as a practice of social exclusion: a review of brazilian academic literature / Moda y trabajo como práctica de exclusión social: una revisión de la literatura académica brasileña

A moda é uma construção social polissêmica que pode implicar exclusão social a diferentes atores sociais. Dessa forma, objetivou-se identificar os impactos da indústria da moda nas construções identitárias, contextos de trabalho e organização social, com o intuito de fomentar práticas psicossociais capazes de enfrentar a ocorrência de exclusão social nesses espaços. Foram identificados nove trabalhos sobre o tema por meio de busca na base de dados SciELO (Scientific Eletronic Library Online). Os resultados demonstraram que os estudos foram produzidos predominantemente por meio de método qualitativo com colaborações interdisciplinares entre ciências humanas e sociais aplicadas. De maneira geral, também evidenciaram a moda associada aos contextos laborais precarizados, permeados por anulação identitária de minorias como mulheres, indígenas e imigrantes, os quais tendem a ser submetidos às práticas psicossociais insatisfatórias que devem ser aprimoradas por meio da promoção de ideário equitativo e práticas inclusivas em múltiplos contextos.

Fashion is a polysemic social construction and may imply social exclusion to different social actors. For this reason, we aimed to identify the impacts of the fashion industry on personnel identity, work contexts and social organization, with the intention of fostering psychosocial practices capable of lowering the occurrence of social exclusion. We retrieved nine papers by the Scientific Electronic Library Online (SciELO) database. We found that the studies were produced predominantly through the qualitative method with interdisciplinary collaborations between the humanities and social sciences. In general, we also realized the fashion industry associated with precarious work contexts, permeated by the identity annihilation of minorities such as women, indigenous and immigrants, who tend to be subjected to unsatisfactory psychosocial practices that must be improved through the promotion of equitable ideas and inclusive practices in multiple contexts.

La moda es una construcción social polisémica que puede implicar exclusión social a diferentes actores sociales. De esta forma, se objetivó identificar los impactos de la industria de la moda en las construcciones identitarias, contextos de trabajo y organización social, con el propósito de fomentar prácticas psicosociales capaces de enfrentar la ocurrencia de exclusión social. Se identificaron nueve trabajos sobre el tema por medio de búsqueda en la base de datos SciELO (Scientific Eletronic Library Online). Los resultados demostraron que los estudios fueron producidos predominantemente por medio del método cualitativo con colaboraciones interdisciplinarias entre ciencias humanas y sociales aplicadas. De manera general, también evidenciaron la moda asociada a los contextos laborales precarizados, impregnados por anulación identitaria de minorías como mujeres, indígenas e inmigrantes, los cuales tienden a someterse a las prácticas psicosociales insatisfactorias que deben ser mejoradas por medio de la promoción de ideario equitativo y prácticas inclusivas en múltiples contextos.

Percepciones del tatuaje como expresión cultural en AMBA / Perceptions about Tattoo as a cultural expression in AMBA

Resumen El tatuaje representa un fenómeno presente desde los comienzos de la historia de la humanidad. A lo largo de los años, supo ocupar diferentes roles asociados a distintos significados y utilidades, y se ha convertido en la actualidad en una forma de expresión de los individuos. En este contexto, el objetivo planteado para este estudio fue descubrir las percepciones del tatuaje como elemento cultural. Para alcanzarlo, se llevó a cabo una investigación descriptiva, de diseño transversal simple, mediante un cuestionario online estructurado y autoadministrado. La muestra se realizó mediante el muestreo bola de nieve y se compuso de 745 individuos que vivían, estudiaban o trabajaban en AMBA (Área Metropolitana de Buenos Aires, Argentina) y tenían entre 18 y 60 años. Como principal resultado de esta investigación se destaca la existencia de fuertes correlaciones entre las principales variables culturales del tatuaje (moda que vino para quedarse; prejuicios en el entorno laboral; expresión cultural). Adicionalmente, fue posible desarrollar un modelo predictivo para la percepción de que el tatuaje representa una expresión cultural, a través de las otras dos variables culturales mencionadas. Por último, se evidencia la existencia de diferencias significativas que permiten reconocer que los individuos más jóvenes presentan un mayor nivel de acuerdo con la idea de que los tatuajes son una moda que vino para quedarse.

Abstract Tattoo represents a phenomenon present since the beginning of human history. Over the years, it took different roles associated with different meanings and utilities, becoming today a form of expression of individuals. Nowadays, tattoos are understood as fashion ornaments, body art, among others, which integrate a social reality. The individuals who are tattooed do not perceive their body as an organic entity, but as an expressive and aesthetic one: they permanently record a story that tells their experiences, feelings and their significant links. Consequently, the tattoo is not a part of the body of the subject, but a synthesis of it. Tattoos are also understood as elements that represent collective identities. In this context, the objective of this study was to discover the perceptions of tattoos as cultural elements. To achieve this, a descriptive research was carried out, with a simple transversal design, through a structured and self-administered online questionnaire. The sample was collected by snowball sampling and consisted of 745 individuals who live, study or work in AMBA (Metropolitan Area of Buenos Aires, Argentina) and who are between 18 and 60 years old. As the main results of this research, it is relevant to highlight the existence of strong correlations between the main cultural variables of tattoos (Fashion that will last; Prejudices in the workplace; Cultural expression). Additionally, a multiple linear regression model was formulated to predict the perception that tattoos are a cultural expression through the idea that it represents a fashion that came to stay and the perception that there are prejudices in the work environment regarding the use of tattoos. Finally, there is evidence of significant differences that allow to recognize that younger individuals have a higher level of agreement with the idea that tattoos are a fashion that will last. These results contribute to previous studies on the subject. Tattoos currently represent elements associated with fashion and culture, which can be reflected in the correlations found between cultural variables, reinforcing previous studies, who add to this panorama the idea that Tattoos constitute a social reality. Moreover, the predicted model found allows to assess the existence of a subculture around the use of tattoo as a cultural expression. Additionally, the importance of the Prejudice variable in the work environment as one of the predictors of the Cultural Expression variable can be evidenced in previous studies that confirm the existence of these prejudices.

Qualidade nutricional de dietas da moda veiculadas em revistas não científicas / Nutritional quality of fad diets in non-scientific journals

Objetivo: o presente estudo teve por objetivo analisar a qualidade nutricional de cardápios de dietas da moda veiculadas nas revistas de circulação não cientificas "Malu" e "7 Dias com você". Métodos: tratou-se de um estudo quali-quantitativo que avaliou cardápios das revistas mencionadas anteriormente, publicadas entre 2015 e 2016. Para a análise do conteúdo nutricional, foi utilizado o software DietWin® para averiguar o valor energético, macronutrientes, o teor de fibra bruta e a quantidade de cálcio, ferro e folato; e para a análise qualitativa, utilizaram-se as recomendações do Guia Alimentar para a População Brasileira para oferta de frutas, hortaliças e a presença de alimentos ultraprocessados. Resultados: as dietas analisadas são hipocalóricas; os macronutrientes estão adequados segundo os intervalos de distribuição de macronutrientes para adultos, pois são deficientes em fibras, cálcio, folato e ferro. Em relação à Ingestão Dietética Recomendada, atendem às recomendações para o consumo de hortaliças, são inadequadas em relação ao consumo de frutas e prescrevem alimentos ultraprocessados. Conclusão: a inadequação do conteúdo nutricional das dietas leva a prejuízos nutricionais e estão relacionadas às carências e aos excessos alimentares. Portanto, faz-se necessária a prescrição de dietas individualizadas por meio de avaliação detalhada.

Objective: This study aimed to analyze the nutritional quality off addiets menus published in the non-scientific magazines "Malu" and "7Days with You". Methods: This was aquali-quantitative study that evaluated menus ofthe previously mentioned magazines published between 2015 and 2016. For the analysis of nutritional content DietWin® software was used to ascertainthe energy value, macronutrients, crude fiber content and the amount of calcium, iron and folate and for qualitative analysisit wasused the recommendations of the Food Guide for the Brazilian Population for the supply of fruits, vegetables and the presence of ultra-processed foods. Results: The diets analyzed are hypocaloric, the macronutrients are adequate according to the Acceptable Macronutrient Distribuition Range, are deficient in fiber, calcium, folate and iron in relation to the Recommended Dietary Intake, meet the recommendations for the consumption of vegetables, are inadequate in relation to the consumption of fruits and prescribe ultra-processed foods. Conclusion: The inadequacy of the nutritional content of diets leads to nutritional losses and are related to deficiencies and overeating. Therefore, it is necessary to prescribe individualized diets through detailed evaluation in a physical consultation.

Ciclos de atenção a dietas da moda e tendências de busca na internet pelo Google trends / Cycles of attention to fad diets and internet search trends by Google trends

Resumo Este trabalho objetivou descrever e analisar conteúdos de notícias associadas a ciclos de buscas ao Google ligados a 8 tipos de dieta: a cetogênica; da lua; da proteína; da sopa; detox; dos pontos; paleo; e Dukan. Estas foram selecionadas por terem sido apontadas pelo Google Trends® (GT) como as mais frequentemente associadas ao termo dieta. Os conteúdos dos sites vinculados aos maiores picos de buscas foram apontados pelo Google News®. O volume de buscas às dietas foram estimados pelo GT com filtros de região (Brasil) e temática (Saúde) considerando o período de 01/01/2012 a 01/01/2017. Os acessos retratam ciclos efêmeros com centenas de picos e quedas de interesse pelas 8 dietas da moda. A ênfase se concentra nas orientações para rápida perda de peso em prol de um ideal de saúde tido como proxy de um ideal estético. A dimensão dos riscos associados às dietas mais restritivas é citada frequentemente e validada por informações de especialistas. Destaca-se a exposição e a influência da opinião de celebridades relatando suas experiências dietéticas. Ao contrário do ideal de variedade e equilíbrio, as dietas da moda retratam a pressão pelo autocontrole alimentar como recurso à construção de um corpo idealizado e tipificado por imagens das celebridades.

Abstract This work aimed at identifying, describing and analyzing news content associated with Google search cycles linked to eight types of diet: ketogenic; moon; protein; soup; detox; points; paleo; and Dukan. They were selected because they were pointed out by Google Trends® (GT) as the most often associated with the term "diet". The content of sites linked to the highest search peaks was pointed out by Google News®. The volume of search attempts for diets was estimated by the GT with region filters (Brazil) and theme (Health) considering the period from 01/01/2012 to 01/01/2017. Results: hits portray ephemeral cycles, with hundreds of peaks and declining interest in the eight fad diets. The emphasis is on guidelines for quick weight loss in favor of optimal health as a proxy for an esthetic ideal. The scale of the risks associated with the more restrictive diets is often quoted and validated by expert information. The exposure and influence of the opinion of celebrities reporting their dietary experiences stand out. Contrary to the ideal of moderation, variety, and balance, fad diets portray the pressure for food self-control as a resource for building an idealized body typified by celebrity images.

Representações LGBTQIA+ e estudos culturais: invisibilidades da diversidade de gênero em audiovisuais publicitários de moda / LGBTQIA+ representations and cultural studies: invisibility of gender diversity in fashionable audiovisuals / Representaciones LGBTQIA+ y estudios culturales: invisibilidades de la diversidad de género en audiovisuales publicitarios de moda

A moda, não raras vezes, é utilizada como marca de distinção social e de apagamentos de gêneros. Mesmo nos dias atuais, sua produção hegemônica distingue os gêneros feminino e masculino e deixa os demais sem representatividade. Por outro lado, a crítica a um modelo de produção publicitário, que robustece os padrões hegemônicos dos binarismos compulsórios entre sexo e gênero, tem sido efetivada por um movimento acanhado e ainda recente que clama por novos modelos de representação. Por meio dos estudos culturais, este ensaio teórico busca refletir sobre as representações LGBTQIA+ nas publicidades de moda, salientando a importância das diversidades de gênero estarem representadas, de forma não estereotipada, dando-lhes visibilidade, evitando seu silenciamento e apagamento.

Fashion is often used as a sign of social distinction and erasure of genres. Nowadays its hegemonic production still distinguishes female and male gender but the others have no representation in it. On the other hand, the criticism about a model of advertising production, which strengthens the hegemonic patterns of compulsory binarisms between sex and gender, has been effected by means of a few expressive and still recent movement that calls for new models of representation. This theoretical essay seeks through cultural studies to reflect on LGBTQIA+ representations in fashion advertising, stressing the importance of gender diversity to be represented in a non-stereotyped way, giving them visibility, avoiding to silence them and their erasure.

La moda se usa a menudo como una marca de distinción social y borradura de géneros. Incluso hoy, su producción hegemónica distingue el género femenino y el masculino y deja otros géneros sin representatividad. Por otro lado, la crítica a un modelo de producción publicitario, que fortalece los patrones hegemónicos de binarismos obligatorios entre el sexo y el género, tiene sido efectuada por medio de un movimiento tímido y aún reciente que exige nuevos modelos de representación. A través de estudios culturales, este ensayo teórico busca reflexionar sobre las representaciones LGBTQIA+ en las publicidades de moda, enfatizando la importancia de que las diversidades de género estén representadas de manera no estereotipada, dándoles visibilidad, evitando su silenciamiento y borradura.

Structure and function of the Fgd family of divergent FYVE domain proteins 1.

FYVE domains are highly conserved protein modules that typically bind phosphatidylinositol 3-phosphate (PI3P) on the surface of early endosomes. Along with pleckstrin homology (PH) and phox homology (PX) domains, FYVE domains are the principal readers of the phosphoinositide (PI) code that mediate specific recognition of eukaryotic organelles. Of all the human FYVE domain containing proteins, those within the faciogenital dysplasia (Fgd) subfamily are particularly divergent and couple with GTPases to exert unique cellular functions. The subcellular distributions and functions of these evolutionarily conserved signal transducers, which also include Dbl homology (DH) and two PH domains, are discussed here to better understand the biological range of processes that such multidomain proteins engage in. Determinants of their various functions include specific multidomain architectures, posttranslational modifications including PIP stops that have been discovered in sorting nexins, PI recognition motifs, and phospholipid-binding surfaces as defined by the Membrane Optimal Docking Area (MODA) program. How these orchestrate Fgd function remains unclear but has implications for developmental diseases including Aarskog-Scott syndrome, which is also known as faciogenital dysplasia, and forms of cancer that are associated with mutations and amplifications of Fgd genes.

Nutritional composition of fad diets published on websites and blogs / Composição nutricional de dietas da moda publicadas em sites e blogs

ABSTRACT Objective To analyze quantitatively the diets available on blogs and websites. Methods The following 15 diets were analyzed: low-carb diet, gluten-free diet and intermittent fasting diet using the Virtual Nutri software program. The nutrients analyzed were as follows: carbohydrates, fibers, proteins, lipids, iodine, sodium, calcium, magnesium, iron, zinc, manganese, phosphorus, selenium, vitamins A, C, B6, B12, D, niacin, and folate. The nutrient values were compared with the Dietary Reference Intake recommendation for adults between the ages of 19-50 years. The macronutrient adequacy percentage was based on a 2000-kcal diet for a healthy life, as recommended by the World Health Organization. Results It was observed that most of the composition of macro and micronutrients was lower than the one established by the World Health Organization and the Dietary Reference Intake recommendation for adults between the ages of 19-50 years. Conclusion Long-term dieting can be a risk factor for several deficiency diseases and may endanger the health of individuals.

RESUMO Objetivo Analisar quantitativamente dietas disponíveis em blogs e sites. Métodos Analisou-se 15 cardápios de dietas intituladas: Low Carb, Dieta Sem Glúten e Jejum Intermitente utilizando o software, Virtual Nutri. Os nutrientes analisados foram: carboidratos, fibras, proteínas, lipídios, iodo, sódio, cálcio, magnésio, ferro, zinco, manganês, potássio, fósforo, cobre, selênio, vitaminas A, C, B6, B12, D, niacina e folato. Os nutrientes tiveram seus valores comparados com recomendações diárias de ingestão das Dietary Reference Intake para adultos da faixa etária de 19 a 50 anos. O percentual de adequação foi feito baseado em uma dieta de 2000kcal para indivíduos saudáveis, de acordo com o preconizado pela Organização Mundial da Saúde. Resultados Observou-se que a maioria dos cardápios teve sua composição de macro e micronutrientes aquém do determinado pela Organização Mundial da Saúde e das recomendações das Dietary Reference Intake para adultos de 19 a 50 anos. Conclusão O seguimento dessas dietas em longo prazo pode ser um fator de risco a diversas doenças carenciais que pode trazer perigos à saúde dos indivíduos.

Modelo de moda: trabalho imaterial e estratégia existencial consumista na expatriação / Fashion model: immaterial labor and existential consumerist strategy on expatriation

Este estudo qualitativo objetivou verificar e analisar a estratégia de viver a vida de modelos de moda, tendo em vista o trabalho imaterial que realizam. Participaram da pesquisa exploratória qualitativa bookers e modelos de moda brasileiros que trabalham ou já trabalharam em situação de expatriação. A coleta de dados ocorreu por meio de entrevista semiestruturada. Os resultados dizem respeito aos sonhos e à inserção na profissão, aos incentivos e aos discursos que fomentam a vida anunciada de modelo; à mobilização de si e incorporação de um padrão de modelo de moda; à ditadura da fita métrica; ao book forte como passaporte internacional; aos percursos e percalços na expatriação; e, por fim, à estratégia de viver a vida na expatriação.

This qualitative study aimed to verify and analyze the strategy of living the fashion models' life, considering the immaterial labor that they perform. Brazilian fashion models who worked or have worked in an expatriation situation participated in this study, and bookers. The data collection occurred through semi-structured interview. The results relate to dreams and the insertion in the profession, the incentives and the speeches that foment the fashion model life announced; to the mobilization of self and incorporation of a model of fashion model; to the tape measure dictatorship; to the strong book as an international passport; to the pathways and obstacles in the expatriation and, finally, the strategy of living the fashion model life in the expatriation.

Um paradoxo entre o existir e o resistir: a moda de viola através dos tempos / A paradox between existing and resisting: viola fashion through the ages

RESUMO A moda de viola é considerada um dos gêneros mais tradicionais da música caipira, se aproximando enquanto gênero narrativo das antigas lendas medievais e dos romances de cavalaria ibéricos renascentistas, sendo ainda afluente da cultura moura. Suas temáticas são reveladoras de uma funcionalidade social que, ao transmitirem valores pedagógicos, morais e expressarem poética e musicalmente determinados contextos sociais, servem como ferramenta de denúncia e crítica a processos de exclusão e opressão social. Este artigo traz uma breve descrição de seus primeiros registros coletados por folcloristas no início do século XX, dos dilemas decorrentes de sua inserção no registro fonográfico a partir de 1929 e das contradições acerca da perpetuação do gênero através dos tempos, focalizando principalmente sua potencialidade no campo dos estudos sobre identidade.

ABSTRACT The moda-de-viola [folk guitar songs] is considered one of the most traditional genres of country music, a narrative genre approaching the old medieval legends and romances of the Iberian Renaissance when it was still an affluent of Moorish culture. Its themes reveal a social functionality that, by conveying pedagogical and moral values, and by expressing poetically and musically certain specific social contexts, serves as a tool to denounce and criticize processes of exclusion and social oppression. This article presents a brief description of the first records collected by folklorists in the early twentieth century, of the dilemmas arising from its insertion in the phonographic record from 1929 onward, and of the contradictions in the perpetuation of this genre over time, focusing mainly on its potentiality in the field of identity studies.

Incomodidad corporal, carga física y nivel de flexibilidad en trabajadores del sector administrativo de una institución de enseñanza superior en Florianópolis, del sur de Brasil / Body discomfort, cargo physical and flexibility in level workers in the administrative sector a higher education institution in southern Brazil

RESUMEN El objetivo de este estudio fue identificar el perfil de los funcionarios de una oficina de una institución de enseñanza superior con relación a las tareas desarrolladas y a sus manifestaciones de dolor, incomodidad corporal. Hicieron parte de este estudio 41 funcionarios que desempeñan sus actividades laborales en posición sentada. Los instrumentos utilizados en la colecta de datos fueron: la escala de Dolor o incomodidad corporal, o método RULA y el banco de Wells. Los datos fueron analizados por medio de estadística descriptiva (media, desviación estándar, frecuencia relativa) y estadística inferencial. En cuanto al análisis de las posiciones de los diferentes segmentos se observó que ninguna posición fue considerada aceptable; en pocas palabras, todas las posturas generan un riesgo a la salud del trabajador. Consideramos de esta manera un perfil negativo de los funcionarios del presente estudio con relación a la presencia de manifestación de dolor, incomodidad corporal, posiciones y sobrecarga biomecánica durante la realización de tareas y nivel de flexibilidad corporal. Que separadamente o en conjunto causan riesgo a la salud del trabajador afectando posiblemente su desempeño y calidad de vida.

ABSTRACT The aim of this study was to identify the profile of the employees of a Secretary of an Education Institution with regard to the complaint of pain and discomfort body. Participants were 41 employees who perform their work activities in sitting on computerized terminals. The instruments used in data collection were: Scale of Pain and/or Discomfort Body, RULA method, Wells's seat. The data were processed using descriptive statistics (mean, standard deviation, relative frequency) and inferential. The analysis of the attitudes of different segments was observed that no position was considered acceptable, it means, all postures generate risk on health of the worker. It is thus a negative profile of employees of this study with regard to the complaint of pain and discomfort body postures and biomechanical overload during task performance and level of body flexibility, which separately or together may cause risks to health worker possibly affecting their performance and quality of life.

Membrane and Protein Interactions of the Pleckstrin Homology Domain Superfamily.

The human genome encodes about 285 proteins that contain at least one annotated pleckstrin homology (PH) domain. As the first phosphoinositide binding module domain to be discovered, the PH domain recruits diverse protein architectures to cellular membranes. PH domains constitute one of the largest protein superfamilies, and have diverged to regulate many different signaling proteins and modules such as Dbl homology (DH) and Tec homology (TH) domains. The ligands of approximately 70 PH domains have been validated by binding assays and complexed structures, allowing meaningful extrapolation across the entire superfamily. Here the Membrane Optimal Docking Area (MODA) program is used at a genome-wide level to identify all membrane docking PH structures and map their lipid-binding determinants. In addition to the linear sequence motifs which are employed for phosphoinositide recognition, the three dimensional structural features that allow peripheral membrane domains to approach and insert into the bilayer are pinpointed and can be predicted ab initio. The analysis shows that conserved structural surfaces distinguish which PH domains associate with membrane from those that do not. Moreover, the results indicate that lipid-binding PH domains can be classified into different functional subgroups based on the type of membrane insertion elements they project towards the bilayer.